For immunofluorescence analysis, U-2 OS cells were fixed and permeabilized for detection of endogenous LATS2 using Anti-LATS2 Recombinant Rabbit Monoclonal Antibody (Product # 703621, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows representative un-treated cells that were stained for detection and localization of LATS2 protein (green) with reduced signal compared to panel e-h) clearly demonstrating enhanced cytoplasmic localisation of LATS2 in U-2 OS cells treated with Nocodozole (200 ng, 24 h). The images were captured at 60X magnification.
For immunofluorescence analysis, U-2 OS cells were fixed and permeabilized for detection of endogenous LATS2 using Anti-LATS2 Recombinant Rabbit Monoclonal Antibody (Product # 703621, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows representative un-treated cells that were stained for detection and localization of LATS2 protein (green) with reduced signal compared to panel e-h) clearly demonstrating enhanced cytoplasmic localisation of LATS2 in U-2 OS cells treated with Nocodozole (200 ng, 24 h). The images were captured at 60X magnification.
Supplier Page from Thermo Fisher Scientific for LATS2 Antibody